Two-photon fluorescence cross-section measurements calibrated with hyper-Rayleigh scattering
نویسندگان
چکیده
The intensity of two-photon-excited fluorescence measured relative to the intensity of hyper-Rayleigh scattering in the same apparatus was used to determine the two-photon-excited fluorescence cross sections of the xanthene dyes Rhodamine B, Rhodamine 6G, and Fluorescein. The measured cross sections at l0 5 1064 nm are s2PF (2) 5 1.5 6 0.3 3 10250 cm4 s, 2.0 6 0.4 3 10250 cm4 s, and 1.4 6 0.3 3 10252 cm4 s, for the three dyes, respectively. The two-photon-excited fluorescence cross section of Rhodamine B was also calibrated with one-photon-excited fluorescence in this apparatus. The result, s2PF (2) 5 2.3 6 0.7 3 10250 cm4 s, is in reasonable agreement with the result calibrated by hyper-Rayleigh scattering. The results for Rhodamine B are 1.5–6 times smaller than previous results calibrated by one-photon-excited fluorescence. © 1999 Optical Society of America [S0740-3224(99)01406-X] OCIS codes: 190.4180, 190.4710, 300.2530, 300.6410.
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